Slit同源物2(Slit2)活性蛋白

[ PROPERTIES ]

Source: Prokaryotic expression. Host: E. coli

Residues: Asn209~Gly374

Tags: N-terminal His-tag

Purity: >95%

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method). Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.05% sarcosyl

and 5% trehalose. Applications: Cell culture; Activity Assays. (May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.8

Predicted Molecular Mass: 19.9kDa

Accurate Molecular Mass: 20kDa as determined by SDS-PAGE reducing conditions.

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex. 

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. 

Store at 2-8^oC for one month. Aliquot and store at -80^oC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37^oC for 48h, and no obvious degradation and precipitation wereobserved. The loss rate is less than 5% within the expiration date underappropriate storage condition. 

[ SEQUENCE ]

[ ACTIVITY ] 

Slit is a family of secreted extracellular matrix proteins which play an importantsignalling role in the neural development of most bilaterians. Humans, mice andother vertebrates possess three Slit homologs: Slit1, Slit2 and Slit3. The Slit2protein has recently been discovered to be associated with the development ofnew blood vessels from pre-existing vessels, or angiogenesis. Slit2 has beenimplicated in promoting angiogenesis in mice (both in vitro and in vivo), in thehuman placenta, and in tumorigenesis. Besides, Gremlin 1 (GREM1) has beenidentified as an interactor of Slit2, thus a binding ELISA assay was conducted todetect the interaction of recombinant rat Slit2 and recombinant rat GREM1. Briefly, Slit2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of100uL were then transferred to GREM1-coated microtiter wells and incubated for2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-Slit2 pAb,then aspirated and washed 3 times. After incubation with HRP labelled secondaryantibody, wells were aspirated and washed 3 times. With the addition of substratesolution, wells were incubated 15-25 minutes at 37 ℃ . Finally, add 50µL stopsolution to the wells and read at 450nm immediately. The binding activity of Slit2and GREM1 was shown in Figure 1, and this effect was in a dose dependentmanner.

[ IDENTIFICATION ]

[ IMPORTANT NOTE ] 

The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.

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