细胞色素P450家族成员1A1(CYP1A1)活性蛋白

[ PROPERTIES ]

Source: Prokaryotic expression. Host: E. coli

Residues: Ser251~His521

Tags: Two N-terminal Tags, His-tag and GST-tag

Purity: >95%

Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.05% sarcosyl

and 5% trehalose. Applications: Cell culture; Activity Assays. (May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.8

Predicted Molecular Mass: 61.5kDa

Accurate Molecular Mass: 62kDa as determined by SDS-PAGE reducing conditions.

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex. 

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. 

Store at 2-8^oC for one month. Aliquot and store at -80^oC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37^oC for 48h, and no obvious degradation and precipitation wereobserved. The loss rate is less than 5% within the expiration date underappropriate storage condition. 

[ SEQUENCE ]

[ ACTIVITY ] 

Cytochrome P450 1A1 (CYP1A1) is a member of Cytochromes P450 superfamilyof enzymes. Cytochromes P450 are a group of heme-thiolate monooxygenases. Itoxidizes a variety of structurally unrelated compounds, including steroids, fattyacids, and xenobiotics. CYP1A1 is also known as AHH (aryl hydrocarbonhydroxylase). It is involved in the metabolic activation of aromatic hydrocarbons(polycyclic aromatic hydrocarbons, PAH). Besides, Heat Shock 70kDa Protein 4(HSPA4) has been identified as an interactor of CYP1A1, thus a binding ELISAassay was conducted to detect the interaction of recombinant rat CYP1A1 andrecombinant rat HSPA4. Briefly, CYP1A1 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to HSPA4-coatedmicrotiter wells and incubated for 2h at 37℃. Wells were washed with PBST andincubated for 1h with anti-CYP1A1 pAb, then aspirated and washed 3 times. Afterincubation with HRP labelled secondary antibody, wells were aspirated andwashed 3 times. With the addition of substrate solution, wells were incubated15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at450nm immediately. The binding activity of of CYP1A1 and HSPA4 was shown inFigure 1, and this effect was in a dose dependent manner.

[ IDENTIFICATION ]

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