凝血因子Ⅶ(F7)活性蛋白
[ PROPERTIES ]
Source: Prokaryotic expression. Host: E. coli
Residues: Ala315~Asp433
Tags: N-terminal His-tag
Purity: >95%
Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.05% sarcosyl
and 5% trehalose. Applications: Cell culture; Activity Assays. (May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 6.4
Predicted Molecular Mass: 14.4kDa
Accurate Molecular Mass: 14kDa as determined by SDS-PAGE reducing conditions.
[ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation wereobserved. The loss rate is less than 5% within the expiration date underappropriate storage condition.
[ SEQUENCE ]
[ ACTIVITY ]
The main role of factor VII (FVII) is to initiate the process of coagulation inconjunction with tissue factor (TF). Tissue factor is found on the outside of bloodvessels-normally not exposed to the bloodstream. Upon vessel injury, tissue factoris exposed to the blood and circulating FVII. Once bound to TF, FVII is activated toFVIIa by different proteases, among which are thrombin (factor IIa), factor Xa, IXa, XIIa, and the FVIIa-TF complex itself. Tissue Factor (TF) has been identified as aninteractor of FVII, thus a binding ELISA assay was conducted to detect theinteraction of recombinant rat FVII and recombinant rat TF. Briefly, FVII werediluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL werethen transferred to TF-coated microtiter wells and incubated for 2h at 37℃. Wellswere washed with PBST and incubated for 1h with anti-FVII pAb, then aspiratedand washed 3 times. After incubation with HRP labelled secondary antibody, wellswere aspirated and washed 3 times. With the addition of substrate solution, wellswere incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wellsand read at 450nm immediately. The binding activity of of FVII and TF was shownin Figure 1, and this effect was in a dose dependent manner.
[ IDENTIFICATION ]
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