胰凝乳蛋白酶C(CTRC)活性蛋白
[ PROPERTIES ]
Source: Prokaryotic expression. Host: E. coli
Residues: Val30~leu268
Tags: N-terminal His-tag
Purity: >95%
Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.05% sarcosyl
and 5% trehalose. Applications: Cell culture; Activity Assays. (May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 5.9
Predicted Molecular Mass: 30.2kDa
Accurate Molecular Mass: 30kDa as determined by SDS-PAGE reducing conditions.
[ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation wereobserved. The loss rate is less than 5% within the expiration date underappropriate storage condition.
[ SEQUENCE ]
[ ACTIVITY ]
Elastase 4 (ELA4) is a member of the peptidase S1 family. The encoded protein isa serum calcium-decreasing factor that has chymotrypsin-like protease activity. Itregulates activation and degradation of trypsinogens and procarboxypeptidases bytargeting specific cleavage sites within their zymogen precursors. Haschymotrypsin-type protease activity and hypocalcemic activity. Besides, Plasminogen Activator, Urokinase Receptor (uPAR) has been identified as aninteractor of ELA4, thus a binding ELISA assay was conducted to detect theinteraction of recombinant rat ELA4 and recombinant rat uPAR. Briefly, ELA4 werediluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL werethen transferred to uPAR-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-ELA4 pAb, thenaspirated and washed 3 times. After incubation with HRP labelled secondaryantibody, wells were aspirated and washed 3 times. With the addition of substratesolution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stopsolution to the wells and read at 450nm immediately. The binding activity of ofELA4 and uPAR was shown in Figure 1, and this effect was in a dose dependentmanner
[ IDENTIFICATION ]
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