内切核酸酶G(ENDOG)活性蛋白
[ PROPERTIES ]
Source: Prokaryotic expression. Host: E. coli
Residues: Met1~Lys294
Tags: Two N-terminal Tags, His-tag and GST-tag
Purity: >92%
Buffer Formulation: 100mM NaHCO3, 500mM NaCl, pH8.3, containing 0.01%
sarcosyl, 5%Trehalose. Applications: Cell culture; Activity Assays; In vivo assays. (May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 9.5
Predicted Molecular Mass: 62.3kDa
Accurate Molecular Mass: 62kDa as determined by SDS-PAGE reducing conditions.
[ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation wereobserved. The loss rate is less than 5% within the expiration date underappropriate storage condition.
[ SEQUENCE ]
[ ACTIVITY ]
ENDOG (Endonuclease G) is a mitochondrial enzyme that cleaves DNA atdouble-stranded (DG)n. (DC)n and at single-stranded (DC)n tracts. This proteinalso has ribonuclease (RNase) and RNase H activities. It has been reported thatEndoG forms complexes with some other proteins, including HSP70 (Heat shock70 kDa protein). Besides, mouse HSPA1A (Heat shock 70 kDa protein 1A) sharessimilarities with rat HSP1A1 in amino acid sequence with the identity of 98.44%. Thus, a binding ELISA assay was conducted to detect the association ofrecombinant rat ENDOG with recombinant mouse HSPA1A. Briefly, ENDOG werediluted serially in PBS with 0.01%BSA (pH 7.4). Duplicate samples of 100uL werethen transferred to HSP1A1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-ENDOG pAb, thenaspirated and washed 3 times. After incubation with HRP labelled secondaryantibody, wells were aspirated and washed 3 times. With the addition of substratesolution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stopsolution to the wells and read at 450nm immediately. The binding activity ofENDOG with HSP1A1 was shown in Figure 1 and this effect was in a dosedependent manner.
[ IDENTIFICATION ]
[ IMPORTANT NOTE ]
The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.
特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!以实际收货产品说明书为准,网站说明书仅供参考。