anti-DNA, AC-30-10, mouse mab

Short overview 

Cat. No. 61014_1 

Concentration 100 µg/ml after reconstitution with 1 ml dist. water 

Product description 

Clone：AC-30-10 

Immunogen：Double- and single-stranded DNA 

Formulation：Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSAin PBS buffer, pH 7.4) 

Applications 

Dot Blot：Assay dependent (on nitrocellulose membrane, after baking at 70°C) 

Immunocytochemistry (ICC)：1:200 (0.5 µg/ml) 

Immunohistochemistry (IHC) - frozen：1:10 (10 µg/ml) 

Immunohistochemistry (IHC) - paraffin：1:10 (10 µg/ml, microwave treatment recommended) 

Background：AC-30-10 stains DNA present in the nuclei and the mitochondria of cells and tissues. It is also a reliable positive control in assays for detection ofDNA antibodies, especially for the detection of autoantibodies in autoimmune diseases. Due to the high sensitivity it can also be used for thedetection of mycoplasma contamination in cell cultures.Binding characteristics: reacts with all forms of native and denatured DNA; reactive alsowith lambda DNA and synthetic DNA.Tested cultured cell lines: XLKE-A6 (Xenopus), RVF-SMC, PTK-2, HeLa.