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pDsRed2-Mito 载体 KL-11120VT

pDsRed2-Mito 载体

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¥ 1080.00 /瓶
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库存46瓶
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pDsRed2-Mito 载体
基本信息

质粒类型:荧光蛋白报告载体
启动子:CMV
克隆方法:多克隆位点,限制性内切酶
载体大小:4.7kb (查看载体序列)
载体抗性:Kanamycin (卡那霉素)
筛选标记:新霉素(Neomycin)

质粒图谱
2022030201495469456

载体描述

pDsRed2-Mito is a mammalian expression vector that encodes a fusion of Discosoma sp. red fluorescent protein (DsRed2; 1, 2) and the mitochondrial targeting sequence from subunit VIII of human cytochrome c oxidase (Mito; 3, 4). The Mito sequence is fused to the 5'-end of DsRed2, a human codon-optimized DsRed variant that is engineered for faster maturation and lower nonspecific aggregation (1, 5). The Mito sequence targets the Mito-DsRed2 fusion protein to the host cell’s mitochondria.


To drive expression of Mito-DsRed2, this vector contains the immmediate early promoter of cytomegalovirus (PCMV IE ). SV40 polyadenylation signals downstream of the DsRed2 gene direct proper processing of the 3'-end of the Mito-DsRed2 mRNA. This vector also contains an SV40 origin for replication in any mammalian cell line that expresses the SV40 T-antigen, a pUC origin of replication for propagation in E. coli, and an f1 origin for single-stranded DNA production. A neomycin resistance cassette—consisting of the SV40 early promoter (PSV40e), the neomycin/kanamycin resistance gene of Tn5 (Neor/Kanr), and polyadenylation signals from the herpes simplex virus thymidine kinase (HSV TK poly A) gene—allow stably transfected eukaryotic cells to be selected using G418 (6). A bacterial promoter (P) upstream of this cassette drives expression of the gene encoding kanamycin resistance in E. coli.

仅供科研使用,不得用于临床检验及治疗使用。

 


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